Advanced Molecular Methods in Biotechnology
| Code | School | Level | Credits | Semesters |
| BIOS4127 | Biosciences | 4 | 20 | Autumn UK |
- Code
- BIOS4127
- School
- Biosciences
- Level
- 4
- Credits
- 20
- Semesters
- Autumn UK
Summary
The ability to manipulate genetic information is central to biotechnology. This module provides in-depth understanding of manipulating cells and biological processes together with the application of this knowledge to genetic engineering.
Practical methods involve isolating, characterising and functionally analysing genes. We are currently in an era of ‘’Omics’’. Over the past few years, major developments have been made regarding the study of genomes. With the advent of next generation and third generation sequencing, whole genome sequences of many species are now available. Such information is revealing a high degree of similarity and conservation between different species and organisms, which in turn is revolutionising the way in which gene function analysis is carried out. An extensive range of post-genomic technologies have been established based on this information and these are revolutionising the analysis that is possible.
Case studies will be presented detailing how different approaches have been used to study genomes and how such developments are influencing the way genetic analysis and biotechnological improvement can be made. Particular emphasis will be paid to the importance of bioinformatics in the study of genomes and their expression. Hands-on experience of these approaches will be provided via problem-based lab and computer training sessions. The methods explained by means of lectures, tutorials and practicals are applicable to plant, microbial and animal cells.
Target Students
Only available to MSc Biotechnology students.
Classes
Blended learning approach of lectures, practicals and tutorials.
Assessment
- 35% Coursework 1: Lab Report based on the transcriptomic data analysis (2,000 words)
- 15% Coursework 2: Lab report based on practical
- 50% Exam 1 (3-hour): Exam
Assessed by end of autumn semester
Educational Aims
To introduce the student to modern molecular methods in biotechnology.Particular focus will be on transgenic methods and the use and application of bioinformatics in understanding the functional roles of genes, metabolites and proteins and how they can be analysed using a variety of new technologies, e.g. deep sequencing, proteomics, mass spectrometry and transcriptomics.Students will gain knowledge in applying, analysing and manipulating cells using current molecular methods and high-throughput technologies. Aspects of systems and synthetic biology will be introduced to understand the whole cell systems approach using genomics, proteomics and metabolomics.Learning Outcomes
1. To manipulate bacterial cells using different cloning and transformation methods.
2. To demonstrate knowledge and understanding of the fundamental principles and practices that underpin genomics, transcriptomics and proteomics and their biotechnological applications.
3. To evaluate current research in -Omics and related biotechnological disciplines.
4. To acquire substantial quantities of information systematically and process it selectively and effectively.
5. To discriminate between the different approaches and techniques used in genomics, transcriptomics and proteomics.
6. To develop ideas and opinions using information from a wide variety of sources.
7. To effectively communicate knowledge of different -Omic technologies and their applications.